The Company is focused on respiratory diseases. Our goal is the
detection of chemicals entities as potential news drugs in biological
fluids and tissues, as lung, bronchus and trachea after administration to
animal. The sample preparation involves homogenization of tissues,
most of the times, whole organ hence the trials with
Precellys®24 Dual – 7 ml vial.
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The analyte levels in the sample are almost the same, so no difference
involving the two homogenizing techniques are detectable (See Figure 1).
Reproducibility is good in both cases, taking into account the
individual variability. Few residual cartilaginous fragments (probably
bronchus/trachea) were observed in both methods (fewer amounts with
Ultraturrax). The results show that there is no impact on the
analyte recovery from matrix. With both methods, temperature
within the sample after processing could reach 70°C depending on the
sample and protocols. Considering the stability of the analyte and the
short time of exposure to this temperature (sample were preserved
in ice before and after processing), sample degradation was not
significant, but a refrigerating device could be useful in different
situation. Ultraturrax is fastidious and time consuming with
cleaning steps and potential contamination between samples. The spent time
is at least 5 times less using Precellys®24 Dual.